First you cannot normally expect two in the same AU have the same
structure, so an over-weighted averaging may cause bias.
It is likely that a rigid-body refinement down to two molecules or
even domains may help improve in your case.
On Jan 16, 2008, at 8:47 AM, Sun Tang wrote:
Hello Everyone,
I have a question about how to improve the electron density. I have
two indepedent molecules in the asymmetric unit (1.9 A resolution).
I solved the structure with PHASER and refined with CCP4i (Rfree =
0.29).
For the first molecule, the density is very good, but for the
second one, the density is much worse than the first one. Are there
any ways to improve the density of the second molecule, such as
some kinds of averaging? Why that happens?
Thank you very much for your help!
Sincerely,
Sun Tang
[EMAIL PROTECTED]
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Lijun Liu, PhD
Institute of Molecular Biology
HHMI & Department of Physics
University of Oregon
Eugene, OR 97403
541-346-4080
