Hi Mike Sometimes keratin from your skin (fingers, nails, etc) appear in the gels as a band around 48-52 kDa. Take care in the handling of the electrophoresis should be a solution.
Other possible reason could be a contamination of the sample loading buffer, also with keratin or with other proteins. Hope it helps Cheers Francisco > Hello, > > When we develop our gel we recently keep getting a horizontal line of > stain > at 50000 mol weight in all lanes of our gel. (This is not a feature of > interest except that the protein that we are interested happens to be > 50000 > mol weight). I would appreciate any ideas on how we can get rid of this > line. > > Thanks. Mike > ----------------------------------------- Francisco J. Enguita, Ph.D. Host-pathogen Interactions Group Macromolecular Crystallography Laboratory ITQB EAN, Av. da República 2781-901 Oeiras Portugal Phone : +351-21-4469663 Fax : +351-21-4433644 E-mail : [EMAIL PROTECTED] -----------------------------------------
