Dear all, Recently, I got a crystallization condition as 0.1 M Bis tris ph 6.5, 1.3Mlithium sulfate, 0.1M NaCl, the shape of crystals is needle cluster, very difficult to grow bigger, microseeding does not work, then I tried macroseeding, and found crystal can grow bigger and rod like. However as for the cryoprotectant, crystal will dissolve in glycerol, ethylene glycol, MPD and PEG, even in the very low concentration about 5%. I found my crystal can grow under the additive 2-propanol in addition to the above mentioned crystallization condition. I also noticed that Hampton cryoprotectant kit has the 2-propanol as cryoprotectant. I want to know if anyone have ever used 2-propanol as the cryoprotectant and what effect it might be. By the way Hampton also mention lithium sulfate can be used as cryoprotectant, the recommended maxium concentration is 2M, I tried and found ice ring is severe and it is very easily to form lithium sulfate salt crystal at this high concentration, I also tried sodium malonate, the diffraction gave me few spots, resolution is only about 6--7 angstrom.
Does anyone encounter such problem by lithium sulfate? Welcome to any replies. Thanks a lot in advance. Donghui
