There's an even faster reagent for staining - takes abut 1 minute overall
and the results look just like dear old Coomassie. This reagent works
wonderfully for us. Yes, the recipe is proprietary - but if you add up the
cost of reagents for normal Coomassie and compare - this one isn't much more
expensive.
http://www.novexin.com/products/InstantBlue.html
Sounds like a good old Bradford reagent AKA colloidal Coomassie G-250.
60 mg G-250 dissolved in 50 ml ethanol and diluted into 1 L of
1/10 dilution of concentrated phosphoric acid (~8.5% final).
"Solubilizing agents" is a smoke screen, for G-250 is fully soluble
in ethanol and for the colloid particles to not penetrate the gel
pores inclusion of any solubilizing agents will only be detrimental.
Betcha it's a browish solution that forms blue pellet when
centrifuged hard.
If so, the claimed 5-10 ng sensitivity can only be achieved
after 1) at least 15 min staining (30 min better; no one abolished
laws of physics that requre diffusion into the gel for the
interaction to take place), 2) some destaining to get rid of
slight brown-blue background.
Dima
