One other staining method deserves to be mentioned with the others
already in this thread:
We use standard issue Coomassie Blue staining, but do the staining and
destaining with heated solutions (place the gel in a tray on a hotplate
set to ~90drg in a fumehood). We routinely have the result after 10-15
minutes (5 min stain, 5-10 min destain).
-bjørn
--
Bjørn Pañella Pedersen
Ph.D Student, MSc
Department of Molecular Biology Office: +45 89425021
University of Aarhus Lab: +45 89425010
Gustav Wieds Vej 10c Email: [EMAIL PROTECTED]
DK-8000 Aarhus C Lab WWW: http://www.bioxray.dk
Denmark
Jacob Keller wrote:
Dear Crystallographers:
just to share something I found out recently, that is really helpful to
know:
5 min Copper Stain protocol
Normal SDS-PAGE gels can be stained in 5 min with 300mM CuCl2. Simply
remove the gel, rinse ~10s in water, place 5 min in CuCl2, and the gel
is negatively stained, with protein bands appearing clear, and
background opaque white. To visualize, I put it on a flatbed scanner in
a darkened room. It works in my hands every bit as well as coomassie (at
least as sensitive, if not more), and if you want, you can stain
afterwards in coomassie with the usual effect. To destain, place gel in
EDTA. The original paper is Lee et al, Anal. Biochem. 1987. Instead of
waiting hours, you can see the results in 5 min with copper! Also, there
is no smell, and it can be re-used, to a point. Gels are stable in H2O
for months, say Lee et al.
Jacob
p.s. I am not in copper comodities...
p.p.s. if anybody knows of down-sides to this, please let me know.
