Both molecules are bound to the same piece of DNA and the DNA is along
the b-axis. Just drop one of the DNA duplexes.
James
On Mar 10, 2008, at 12:54 AM, Sabine Schneider wrote:
Hello everyone,
I am working on the structure of a protein-DNA complex with 2 mol/
ASU. Data are to 2.8A and the symmetry appears to be orthorhombic
(P212121; a=42.280 b=113.340 c=134.670) I solved the structure by
moleculare replacement using the coordinates of the protein in
phaser. I got relative good density for the DNA and started building
for the DNA for one protein molecule and gave the model back to
phaser. The statistics for the solution are very good (RFZ=16.7
TFZ=27.8 PAK=0 LLG=820 RFZ=14.9 TFZ=42.9 PAK=0 LLG=2215 LLG=2215).
My problem is now that the ends of the DNA strands of the two
complexes in the asu are crashing by overlapping almost perfectly as
well as with the symmetry related molecules. So instead of beeing
able to fit in a 15mer, which was used in the crystallisation, I
only can fit in maybe an 8mer (which is also not making really sense
DNA-sequence wise). I tried reindexing to klh and lhk but same
problem.
Has anyone an idea how to solve this?
Thanks a lot for your help!
Sabine
--
James Stroud
UCLA-DOE Institute for Genomics and Proteomics
611 Charles E. Young Dr. S.
Los Angeles, CA 90095
http://www.jamesstroud.com
