Looks ok I guess.. for the highest shell, if Rmerge is less than 0.45 and I/sigma is about 2, it is worth a try.. as James said, completeness might be from why it is incomplete.. is it something like C2?
experts might tell us more.. Best, Partha On Mon, Mar 17, 2008 at 11:03 AM, Melody Lin <[EMAIL PROTECTED]> wrote: > well, redundancy for the highest shell is 4.8, I/sigma is 3, Rmerge for > overall is 0.08 for highest shell is 0.336. I/sigma and Rmerge don't seem > quite nice... > > thanks. > > > > On Mon, Mar 17, 2008 at 11:51 AM, Partha Chakrabarti <[EMAIL PROTECTED]> > wrote: > > > Hi Melody, > > > > There was a nice discussion in this year's ccp4 study weekend. In > > general, one needs to consider several factors.. If you were at 3A, or > > low symmetry, you would of course try to get the maximum out of it, on > > the other hand, there are requirements for experimental phasing.. in > > general, judge it from: > > > > 1. Completeness > > 2. Redundancy > > 3. I / Sigma > > 4. R merge statistics > > > > Not just one of them. If you are pushing it too far, you will see the > > effect in later refinement step.. > > With 74% completeness, how does the other parameters look like? > > > > HTH, Partha > > > > > > > > > > > > On Mon, Mar 17, 2008 at 10:06 AM, Melody Lin <[EMAIL PROTECTED]> wrote: > > > Hi all, > > > > > > I have always been wondering... for a data set diffracting to say 2.15 > > > Angstrom but in the highest resolution shell (2.25-2.15) the > completeness is > > > 74%, should I use merge all the data and call it a 2.15 A dataset or I > > > should cut the data set to say 2.25 A where the highest resolution shell > has > > > better completeness (>85%)? What is an acceptable completeness value for > the > > > highest resolution shell? > > > > > > Thank you. > > > > > > Best, > > > Melody
