We have had good experience with the awfully simple minded approach
of using two pET vectors with different antibiotic resistance.
Its the easiest thing to do, and it often works ...
Apologies for the shameless plugin, since there are many good papers
on the subject, but you can read some hints and case studies at:
http://scripts.iucr.org/cgi-bin/paper?S0907444906031003
(its Open Access)
A.
On 2 Apr 2008, at 19:39, P Hubbard wrote:
Hi,
If you are expressing just two proteins, you could try a single pET
vector with a pCDF vector. The only reason I'm suggesting this is
that I had trouble with pET-Duet, but doing each one separately
worked first time (plasmid size issue?). I used pET24-a and pCDF-1b
- so I had one construct untagged, and the other with a cleavable
His-tag.
Cheers
AGS
Date: Wed, 2 Apr 2008 15:55:27 +0200
From: [EMAIL PROTECTED]
Subject: [ccp4bb] Co-expression plasmids
To: [email protected]
Dear All,
Anyone have experience with the NovaGen Duet co-expression vectors?
Or can recommend others?
http://www.emdbiosciences.com/html/NVG/Duet_Spot.html
Greetings,
Mark
Mark J. van Raaij
Dpto de Bioquímica, Facultad de Farmacia
Universidad de Santiago
15782 Santiago de Compostela
Spain
http://web.usc.es/~vanraaij/
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