Narayanan Ramasubbu a écrit :
Dear all:
I am noticing that in some of my structures, at 1.5 A resolution, 1)
there is some negative density around the C of the carboxyl groups.
2) I also notice the negative density around S in a disulfide region.
It is as though the disulfide is broken.
Could some body enlighten me on this feature?
The other original reference for the radiation damage effect on carboxyls
is actually:
Burmeister, W. P.
Structural changes in cryo-cooled protein crystals owed to radiation damage.
Acta Cryst. D 56, 328-341 (2000).
Greetings
Wim Burmeister
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Wim Burmeister
Professeur, Membre de l'Institut Universitaire de France
Unit of Virus Host Cell Interactions (UVHCI) UMR5233 UJF-EMBL-CNRS
6 rue Jules Horowitz
B.P. 181, F-38042 Grenoble Cedex 9 FRANCE
E-mail: [EMAIL PROTECTED]
Tel: +33 (0) 476 20 72 82 Fax: +33 (0) 476 20 94 00
http://www2.ujf-grenoble.fr/pharmacie/laboratoires/gdrviro
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