Hi Wei Yong, On May 12, 2008, at 4:12 PM, Yong, Wei wrote:
I have solved a pig mitochondrial protein crystal structure whose molecular weight is about 95kd. However, I do not have the sequence information of this protein. It is weird that I could not blast its sequence in pig genome. Because pig sequences of known proteins are very similar to their human homologous, I used this protein's human homologous sequence to fit into electron density. Currently, I am planning to sequence the full length sequence of the protein. However, I was not able to successfully extract mRNA from pig liver using Qiagen RNeasy kit (I only tried once). Does any body have ideas about how to extract mRNA from pig live (or animal tissue in general)? Are there any other database I can search for pig protein sequence information?
You can use the Copenhagen PigEST database. http://pigest.ku.dk/server/index.html Cheers, Martin . B. Martin Hallberg, PhD Assistant professor Department of Cell and Molecular Biology Medical Nobel Institute Karolinska Institutet Nobels väg 3 SE-171 77 Stockholm Sweden
