Hi,
This topic is not related to CCP4. I am having problem of crystallizing
one protein. It's a pretty small protein with size around 15kDa. I have
stock concentration around 100mg/mL. Crystallization plates I set up are
with concentration of 10mg/mL, 30mg/mL and 50mg/mL. All the plates had been
set up at least one week. Only around 5 wells per plate or less formed
precipitation. The rest of wells are pretty clear still. Is there any
suggestion for reducing protein solubility or increasing the chance of
getting crystals?
Thanks for your time,
Jennifer
