parkash wrote:
Hi,
I have one structural refinement problem.
I am working on a protein crystals which diffracted to 2.8 Å. But when
I refine through REFMAC5, with 0.1 wt(geometry to x-ray terms), I get
high B-factors around 70. But if I do TLS refinement, the R-factors
lower down and B-factors come down to 40.
My question is; Are TLS refined B-factors real?? Can I trust them?
or what else could be done to lower down the B.factors?
BR
vimal
This is or should be a FAQ - all B factors after TLS are relative to the
TLS restraints
Try running TLSANL and that should give you back a more meaningful
overall B
Eleanor
