Hi,
It would be nice if you mention more clearly how you have done
TLS..i.e.,domains or individual residues or molecules !! You should keep in
mind that you have 2.8A data. I would do TLSANL with domains or molecules.
Best regards,
Krishna Ch
PhD Student
Hannover Medical school
Germany
On Wed, May 14, 2008 at 11:36 AM, parkash <[EMAIL PROTECTED]> wrote:
> Hi,
> I have one structural refinement problem.
> I am working on a protein crystals which diffracted to 2.8 Å. But when I
> refine through REFMAC5, with 0.1 wt(geometry to x-ray terms), I get high
> B-factors around 70. But if I do TLS refinement, the R-factors lower down
> and B-factors come down to 40.
>
> My question is; Are TLS refined B-factors real?? Can I trust them?
> or what else could be done to lower down the B.factors?
>
> BR
> vimal
>
