Hello,
You may want to have a look at the UV LEDs, which should be the cheapest
option if you only need a specific wavelenth.
I found this on google: http://www.3dzled.com/other.html. It seems that they
can make 280nM LEDs. It is interesting to note that they also said these
LEDs' "Wavelength tolerance is usually within +/- 5 nm. For example 254 nm
would be 249 nm to 259 nm and 415 nm would be 410 nm to 415 nm or 415 nm to
420 nm" - apparently not as pure as those generated by monochrometers, but
should be good enough for quatitating protein or exciting some fluorophores.
I wonder if the microscope makers would ever consider using these instead of
those multi-thousand $ light sources.
One more thing: do not forget that 280nm UV is extremely harmful to human
eyes and skin.
Zhijie Li
----- Original Message -----
From: "Torres-Larios Alfredo" <[EMAIL PROTECTED]>
To: <[email protected]>
Sent: Wednesday, May 14, 2008 7:59 PM
Subject: [ccp4bb] UV light source for protein xtal detection
Dear all,
Here's another non CCP4 question: does anyone know a cheap alternative to
set up a UV source at 280 nm? I'd really like to have one :), but I really
don't have the $20K Dlls needed to buy a UV/white light source from the
crystallographic vendors :(.
Thanks so much in advance for your answers, Alfredo.
Alfredo Torres-Larios, PhD
Assistant Professor
Instituto de Fisiologia Celular, UNAM.
Ciudad Universitaria, Mexico
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