...by lower wavelength, I mean longer wavelength... or lower energy. Take your pick.
Dave 2008/5/22 David Briggs <[EMAIL PROTECTED]>: > Hi Andrew > You don't say what your protein is crystallised in, or has seen during > purification, but maybe S- MERCAPTOCYSTEINE might fit? > (search for CSS in MSD CHEM) > Do you have lower wavelength data that you might get a sulphur > anomalous signal to check this? > > S-methyl Cysteine might fit as well - but the difference peak and bond > lengths look more S-S than S-C. > > Hope this helps, > > Dave > > 2008/5/22 Andrew Gulick <[EMAIL PROTECTED]>: >> Greetings. >> >> I'm wondering if anyone has ever seen something like this. I have a 2.1 A >> data set (synchrotron data) that is nearing completion. I see density that >> appears to join a cysteine side chain to a lysine (30 residues away from >> each other in primary sequence). There is a picture of the density here. >> >> http://labs.hwi.buffalo.edu/gulick/cyslys.html >> >> I have modeled this in as a Cysteine sulfenic acid (Side chain is Cb-Sg-OH) >> and refined. There remains a bit of positive Fo-Fc density above the oxygen >> and the lysine N and sulfenic acid hydroxyl are 1.8A away. >> >> I have some other crazy ideas but haven't been able to find any precedent in >> the literature. Any thoughts on what this might be or if anyone has seen >> something similar would be greatly appreciated. >> Andy >> >> >> >> >> >> -- >> Andrew M. Gulick, Ph.D. >> ----------------------------------- >> (716) 898-8619 >> Hauptman-Woodward Institute >> 700 Ellicott St >> Buffalo, NY 14203 >> ----------------------------------- >> Senior Research Scientist >> Hauptman-Woodward Institute >> Dept. of Structural Biology, SUNY at Buffalo >> >> http://www.hwi.buffalo.edu/Faculty/Gulick/Gulick.html >> http://labs.hwi.buffalo.edu/gulick >> > > > > -- > ============================ > David C. Briggs PhD > Father & Crystallographer > http://www.dbriggs.talktalk.net > AIM ID: dbassophile > ============================ > -- ============================ David C. Briggs PhD Father & Crystallographer http://www.dbriggs.talktalk.net AIM ID: dbassophile ============================
