Dear all,
I'm facing a particular MR problem:
I'm trying to solve the structure of a 20kDa protein (1 mol/AU) in a
cubic SG P432
(according to systematic absences and pointless)
Sequence homology is about 30%.
As I only get 'flat' scores with Phaser in P432, I lowered to SG P4
(with 6 mol/AU)
and get following Z-scores:
RFZ= 7.9 TFZ= 9.9 (LLG=111)
However, when refining this result (Refmac), things do not look
allright at all...?
I'm aware of the fact that in the presence of translational NCS,
Phaser can give large Z-scores.
But when using 6 molecules/AU as a one model in Phaser, I'm in the
feeling that this should be ruled out..??
Has anyone noticed such a case before please..?
Regards
Kristof
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Kristof Van Hecke, PhD
Biomoleculaire Architectuur
Celestijnenlaan 200 F
B-3001 Heverlee (Leuven)
Tel: +32(0)16327477
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