Dear Debajyoti Thank you very much for your help. I input the each peak values as 10 and carried out the rotation and translation function again. I didn't get a improved solution by molrep. can i edit the self-rotaion function and translation function solution file as input ?
best regards, carl soja >Hi, > >I dare to say about the possible way to do molrep from my recent experience. You can >choose "rotation and translation function" job at first and do the self rotation fuction (for >multimer) after that.Each of these run will generate two different outputs *_rf.molrep_rf ans >*_srf.molrep_rf. Make the third run inputting both of these peak values. Give NMON as 4. >You can also use the protein sequence hare. > >Then refine it with Refmac, that again should decrease your R factor. > >I will be highly benefitted if some one point out my mistake. > >regards >Debajyoti > >On Fri, 25 Jul 2008 Carl Soja wrote : >Dear all > >I tried to solve one structure by ccp4i molrep(resolution at 3.0 A, space >group C222, sequence ID 30%). I can get a good Rfactor 0.528 at first >translation function. However, the second translation function Rfac is >0.526, the third is 0.525, the fourth is 0.525. All of the translation >function Rfacs are too closed. I changed the model and minimum resolution >for search, the Rfactor closed no any improved. My structure estmates four >molecules in the aymmetric unit. This is my first time found the closed Rfac >by molrep. From the low translation function Rfac, it seems that it is >correct solution. I checked the solution and found some clashes in the >structure.I am not sure why the Rfactor too closed in next the molecule >search? I know this is unusual solution by molrep. Does it mean the >diffraction data has problem? >Any suggestions are welcome.Thank you in advance! > >Carl soja
