Hi there, I think 55% completeness is insufficient - you really need to collect more data.
Take a look at this movie from James Holton's website: http://ucxray.berkeley.edu/~jamesh/movies/osc.mpeg Stop the movie at 55% and check out how lousy the maps are compared to 95-100% - and I think these maps are generated with "perfect" phases, at 1.5A resolution. Molecular replacement programs and refinement programs are going to struggle with just over half a dataset - this is also why your R-factors are sky high. In addition, I know of cases where reviewers have cast doubt over structures (of a point mutant) with data of 80% completeness. I don't think a 55% complete dataset is going to get out there. I really think more data is the only way our of your predicament. I hope you have a few crystals ready to go! Good Luck, David. 2008/7/27 Carl Soja <[EMAIL PROTECTED]>: > HI all, > > Thank you very much for your kindly answer. > > I also tried to use different program like phaser, amore, and some MR servers. > actualy, I don`t know how many molecule in AU clearly. 2-6 molecules in 76%- > 35% of solvent content. 4mer is 50% of solvent content. the homolgous > structure has high solvent content over 70%. when i used phaser program to > do MR, I can get several solutions but not good structure conformation. I > don`t know how to use phaser do automatic more than 2 molecules search > like molrep program. > As spacegroup, I checked the C222 and C2221. the phaser program can give > both solutions when I changed the search model or maximum solution limit. > > my question is how i can search more than 2 molecules by phaser program like > molrp only input over 2. > > by the way, my diffraction data have low completeness(55%) and low > redandancy(1.8). > > Thanks in advance!! > > carl roja > -- ============================ David C. Briggs PhD Father & Crystallographer http://www.dbriggs.talktalk.net AIM ID: dbassophile ============================
