I feel compelled to add: "rather nice cubic <0.1mm" presumably means
they're 90x90x90um? At any state-of-the-art synchrotron beamline with a
half-way decently-sized beam, that's an absolute stonker, we rarely
optimize more if they're that large.
phx.
Vincenzo Carbone wrote:
Dear all,
I was wondering if anyone had some practical advice in regards to
increasing the size of a crystal. Currently my enzyme forms these
rather nice cubic and very sharp <0.1mm (in 25% peg 5000, 0.1M
ammonium sulphate, 0.1M Tris ph 7.0) crystals following optimisation
of the pH and precipitant concentrations of the initial screening
condition and by playing with various increasing levels of protein
concentration 20-60mg/ml.
Has anyone had success with additive screens in increasing crystal
size and what were they?
Any advice would be useful thanks
Vince
