Hi! I would suggest to try another temperature or seeding. It worked for me before. Good luck! Nurit.
"Anyone who has never made a mistake has never tried anything new. Albert Einstein" Nurit Mirkin Bril> Date: Wed, 10 Sep 2008 08:02:53 -0400> From: [EMAIL PROTECTED]> Subject: Re: [ccp4bb] crystal size> To: [email protected]> > My usual first approach to increasing crystal size is to decrease > precipitant and increase protein concentration, keeping the mixture in > the proper nucleation zone, but providing more protein for crystal > growth. Minimizing dust or particulates by filtration and centrifugation > of reagents and protein may help, by reducing nucleation sites in the > solution. Crystallization at a lower temperature may also result in > fewer, but larger crystals. Or you could try seeding.> > Cheers,> > -- > ------------------------------------------------------------------------> Roger S. Rowlett> Professor> Colgate University Presidential Scholar> Department of Chemistry> Colgate University> 13 Oak Drive> Hamilton, NY 13346> > tel: (315)-228-7245> ofc: (315)-228-7395> fax: (315)-228-7935> email: [EMAIL PROTECTED]> > > Walter Novak wrote:> > Hi Vince,> >> > There is a very nice paper by Monika Budayova-Spano in Acta Cryst 63D > > 2007 339-347. "A methodology and an instrument for the > > temperature-controlled optimization of crystal growth." > >> > Best,> > Wally> >> > On Sep 10, 2008, at 2:23 AM, Vincenzo Carbone wrote:> >> >> Dear all,> >>> >> > >>> >> I was wondering if anyone had some practical advice in regards to > >> increasing the size of a crystal. Currently my enzyme forms these > >> rather nice cubic and very sharp <0.1mm (in 25% peg 5000, 0.1M > >> ammonium sulphate, 0.1M Tris ph 7.0) crystals following optimisation > >> of the pH and precipitant concentrations of the initial screening > >> condition and by playing with various increasing levels of protein > >> concentration 20-60mg/ml.> >>> >> > >>> >> Has anyone had success with additive screens in increasing crystal > >> size and what were they?> >>> >> > >>> >> Any advice would be useful thanks> >>> >> > >>> >> Vince> >> >> > Walter R.P. Novak, Ph.D.> > Postdoctoral Fellow> > Rosenstiel Basic Medical Research Center> > Brandeis University> > 415 South St. MS 029> > Waltham, MA 02454-9110> > Phone: (781) 736-4944> > Fax: (781) 736-2405> >> >> >> >> > - _________________________________________________________________ Stay up to date on your PC, the Web, and your mobile phone with Windows Live. http://clk.atdmt.com/MRT/go/msnnkwxp1020093185mrt/direct/01/
