Dear Amit -- On 30 Sep 2008, at 14:16, amit sharma wrote:
I am trying to crystallize a protein with a peptide attached to it via a 9aa linker. I am mostly getting precipitates in conditions carrying salts. I have tried optimization screens by varying the pH and salt concentrations, but in vain. Can anyone please suggest ways by which I could optimize the conditions further?
Among all the possibilities, you can: - try to crystallize without salt ; - change the temperature ; - dilute your protein ; - add some isopropanol (or other) to make the solution "smoother" ; - change your linker size ; - ... HTH Kind regards. -- Leo -- ------------------------------------------------------------ Chavas Leonard, Ph.D. @ home Research Associate Marie Curie Actions Fellow ------------------------------------------------------------ Faculty of Life Sciences The University of Manchester The Michael Smith Building Oxford Road Manchester Lancashire M13 9PT ------------------------------------------------------------ Tel: +44(0)161-275-1586 e-mail: [EMAIL PROTECTED] http://personalpages.manchester.ac.uk/staff/leonard.chavas/
