Dear All, Many thanks for the suggestions. Cheers, Amit
2008/10/1 Chavas Leo <[EMAIL PROTECTED]> > Dear Amit -- > On 30 Sep 2008, at 14:16, amit sharma wrote: > > I am trying to crystallize a protein with a peptide attached to it via a > 9aa linker. I am mostly getting precipitates in conditions carrying salts. I > have tried optimization screens by varying the pH and salt concentrations, > but in vain. Can anyone please suggest ways by which I could optimize the > conditions further? > > > Among all the possibilities, you can: > - try to crystallize without salt ; > - change the temperature ; > - dilute your protein ; > - add some isopropanol (or other) to make the solution "smoother" ; > - change your linker size ; > - ... > > HTH > Kind regards. > > -- Leo -- > ------------------------------------------------------------ > Chavas Leonard, Ph.D. @ home > Research Associate > Marie Curie Actions Fellow > ------------------------------------------------------------ > Faculty of Life Sciences > The University of Manchester > The Michael Smith Building > Oxford Road > Manchester Lancashire > M13 9PT > ------------------------------------------------------------ > Tel: +44(0)161-275-1586 > e-mail: [EMAIL PROTECTED] > http://personalpages.manchester.ac.uk/staff/leonard.chavas/ > > > -- Amit Sharma, Ph.D. Research Associate, Department of Biology, University of York, YO10 5DD UK
