Hi -
I would not use mlphare for anything marginal (to be honest not at all).
Both SHARP (use eternal pdb file in top page of the gui) and the new
Phaser (look at the doc for scripts for this case) can do what you want.
Tassos
On Nov 4, 2008, at 11:55, Thomas Edwards wrote:
Dear BB,
I would like to ask for some advice on phased molecular replacement
if possible.
I have a MR model that has so far not proved successful with Phaser,
Molrep, Amore, Beast etc.
I have SeMet MAD data to 3.6A that gives decent looking anomalous
difference peaks, looks stable in mlphare, and produces solvent
flattened maps to 2.8A in DM that look like the density might be a
sensible shape - wrt solvent gaps etc - but not interpretable so far
(I will be trying phasing in SHARP, CNS etc).
In the mean time, is there a good way to combine phases that may be
slightly sensible with molecular replacement? Things may also be
complicated by the possibility of NCS translations…
Any advice gratefully received.
Many thanks to all those who continue to provide excellent
suggestions,
Cheers
Ed
______________
T.Edwards Ph.D.
Garstang 8.53d
Astbury Centre for Structural Molecular Biology
University of Leeds, Leeds, LS2 9JT
Telephone: 0113 343 3031
http://www.bmb.leeds.ac.uk/staff/tae/
If you're not part of the solution, you're part of the precipitate.
~Henry J. Tillman
