Here we are dealing with two different state of chemistry, solid state and solution state, If one of the minima in solid state resembles the biological state minimum, then there is a possiblw way to clearly define the biology and its significant interaction of that particular 'mer' of a protein, other wise we end up with pure physical interaction.
But my question is have we answered Wouldn't the high concentration in the crystallization drop further favor dimerization? this part ... S.Jayashankar Research Student Institute for Biophysical Chemistry Hannover Medical School Germany. On Thu, Dec 11, 2008 at 5:53 PM, Phoebe Rice <[EMAIL PROTECTED]> wrote: > Mass action is on the crystal's side. > Two recent examples of proteins that are dimers by standard > solution assays, but form weak/transient/co-factor-dependent > tetramers to function, and those tetramers are seen in the > crystal. (There is good solution data to back up the > relevance of the tetramer in both cases). > > Yuan P, Gupta K, Van Duyne GD. Tetrameric structure of a > serine integrase catalytic domain. Structure. 2008 Aug > 6;16(8):1275-86. > > Mouw KW, Rowland SJ, Gajjar MM, Boocock MR, Stark WM, Rice PA. > Architecture of a serine recombinase-DNA regulatory complex. > Mol Cell. 2008 Apr 25;30(2):145-55. > > Phoebe > ========================================== > ---- Original message ---- > >Date: Thu, 11 Dec 2008 10:09:33 -0600 > >From: "Santarsiero, Bernard D." <[EMAIL PROTECTED]> > >Subject: [SPAM:#] [ccp4bb] O/T: can a protein which dimerizes > in solution crystallize as a monomer? > >To: [email protected] > > > >In parallel with the discussion around this off-CCP4-topic, > are they any > >good examples of the opposite case, where the protein is a > monomer in > >solution (as evident from light scattering, MW determination > through > >centrifugation, EPR, etc.) but crystallizes as a dimer or > higher multimer? > > > >Bernie Santarsiero > Phoebe A. Rice > Assoc. Prof., Dept. of Biochemistry & Molecular Biology > The University of Chicago > phone 773 834 1723 > > http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123 > > RNA is really nifty > DNA is over fifty > We have put them > both in one book > Please do take a > really good look > http://www.rsc.org/shop/books/2008/9780854042722.asp >
