Here's the summary and some own experimental data.
First off all thank you all for your replies.
trick 1: put a glass bottle filled with water between fiberoptics and
the mirrors of your base (Isolde Le Trong)
trick 2: suspend plates to allow air circulation, with bulb under
plate scopes (Jose Antonio Cuesta_Seijo)
Halogen driven fiberoptics tested ∆1.2 F ~ 0.6 ˚C (Matthew Franklin)
Zeiss LED has too much diffuse light and warms up (Clemens Grimm)
Zeiss LED used at Diamond beamlines are very good (Sandy James)
(I'm wondering if both of you had different bases, mine will have a
mirror to focus the LED light where I want it to be)
And here my own tests:
I have a Zeiss Stemi2000 right now mainly to test the LED light, the
LEDs are in the base and can't be moved around. In this case the
contrast & light is sufficient to visualize crystals and mount them.
However the glass above the LED's really gets warm starting
temperature was 20.9 ˚C and after 1 hour it stayed at 26.3 ˚C.
Since the model I will most likely purchase will have a mirror, I
assume heating should not be as dramatic, and keep in mind this will
be only the mounting microscope with 50x magnification. The highend
model I decided to go for fiberoptics, mainly because of brightness to
take pictures etc (100x).
I tried one of our neighbouring labs fiberoptics microscope and there
was an increase over an hour of 0.3˚C compared to the surrounding
environment.
I'm expecting the smaller scope to arrive within the next 2-3 weeks
and I'll send an update on the thermal aspects to the board then.
Hope this information is useful to the community,
Jürgen
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Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Biochemistry and Molecular Biology, W8708
615 North Wolfe Street
Baltimore, MD 21205
Phone: +1-410-614-4742
Fax: +1-410-955-3655
Web: http://faculty.jhsph.edu/default.cfm?faculty_id=2101
