Dear All, Recently I solved a structure at 2.5 A with PHASER, with two molecules in assymetric unit. There are no short contacts in the solution. However, the Rfree stays at about 50% after the refinement of rigid boby and restrained refinement in CNS. The sequences homology is about 30%.
What is the usual way to deal with this kind of problems? Should I just refine
the backbone of the structure and then build the side chains?
Your suggestions are greatly appreciated!!!
Best wishes,
Sun
