Well, what do you expect the anomalous signal contributed from your 45 seleniums in a perfect world to be when the asymmetric unit contains 1300 aa? Do you think a dataset with Rmerge of ~15% is good enough to detect a signal of even 2%? (Note: I did not do the calculation, so I just made up the number of 2%.)

Jim


On Fri, 20 Mar 2009, Kumar wrote:

Hello CCP4bb members,

I have been trying to obtain phases for a protein which contain ~1300aa. We
have obtained native data to a resolution of 3.3A (Space group I222 or
I212121). But we are having tough time phasing it.
...

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