David Cobessi wrote:
Liew Chong Wai wrote:
Hi,
Good day
I am currently building my structure by using COOT. My protein is a
tetramer protein and I have fit my protein sequence into one of the
monomer of the homologous model. May I know how can I replace other
monomer with the amended monomer??
Thanks
*Chong-wai*
Hi Chong-wai,
Several possibilities to generate the tetramer from the monomer, let
say A.
You can calculate the RT matrices ( in CCP4 or using LSQMAN and
MOLEMAN2 or using O for example) to superpose the monomer A onto B, C
and D and then apply these matrices to the amended monomer (A) in
order to generate the tetramer.
You can also superpose the monomer A onto B in COOT by using SSM or
LSQ and save the new position as monomer B in COOT.... etc... and then
you can generate 4 monomers and the tetramer. Do not forget to change
the chain ID before refinement.
Blimey.
Extensions -> NCS -> Copy NCS Chain...
See Section 5.27 of the user manual for more information.
http://www.ysbl.york.ac.uk/~emsley/coot/doc/chapters/user-manual_5.html#SEC137
Paul
