On Apr 1, 2009, at 4:35, Liew Chong Wai wrote:

Hi,

Good day
I am currently building my structure by using COOT. My protein is a tetramer protein and I have fit my protein sequence into one of the monomer of the homologous model. May I know how can I replace other monomer with the amended monomer??
Thanks


I admit that a trick I found useful, is after building one chain do a molecular replacement with this as a model and lookign for four copies. Then go back to Coot ... avoids thinking about rotations matrixes which is always a challenge (for me that is).

Tassos


Chong-wai


P please don't print this e-mail unless you really need to
Anastassis (Tassos) Perrakis, Principal Investigator / Staff Member
Department of Biochemistry (B8)
Netherlands Cancer Institute,
Dept. B8, 1066 CX Amsterdam, The Netherlands
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