Unless you want a ligand free molecule it might be a good idea to add the substance already to the growing Ecoli (?) culture. That has proven to be successful for such molecules.
Best wishes Kornelius On Wed, 1 Apr 2009 16:59:05 +0000 KUMARASWAMI MUTHIAH <[email protected]> wrote: > > Anybody tried to cocrystallize the protein-progesterone or estrogen > complexes, if so how do you go about the solubility of these compounds? > Progesterone is only soluble in 50% chloroform or 100% DMSO and the dilution > of this stock is not possible as chloroform falls out of solution. Lots of > papers out there used soaking with progesterone or expressed the protein in > the presence of progesterone. Any suggestions would be appreciated.Thanks > > > > _________________________________________________________________ > Rediscover Hotmail®: Now available on your iPhone or BlackBerry > http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Mobile1_042009 ---------------------------------------------- Kornelius Zeth Max Planck Institute for Developmental Biology Dept. Protein Evolution Spemannstr. 35 72076 Tuebingen, Germany [email protected] Tel -49 7071 601 323 Fax -49 7071 601 349
