Hi, Could it not be that the NADP site is not fully occupied ? That can (even quite often) happen. Do you see +/-v peaks in the difference map around the NADP binding site ? What was the molar ratio of NADP to protein in your crystallization buffer ?
Cheers, Boaz ----- Original Message ----- From: sajid akthar <[email protected]> Date: Sunday, August 9, 2009 14:59 Subject: [ccp4bb] To: [email protected] > Hi all > > I have used NADP in my crystallisation condition; and the > crystal diffracted well; I built the model completely and the r- > factor and r-free are 0.26 and 0.29 respectively. The resolution > is 2.3A. I am looking for NADP, in the binding region; I could > see some density for NADP; But it is not continuous; there is no > density for the middle phosphate group in fo-fc as well as 2fo- > fc. Is it possible that NADP could hydrolyse? if so what could > be the hydrolysed product > > > thank you > > sajid > > > > Looking for local information? > Find it on Yahoo! Local http://in.local.yahoo.com/ > Boaz Shaanan, Ph.D. Dept. of Life Sciences Ben-Gurion University of the Negev Beer-Sheva 84105 Israel Phone: 972-8-647-2220 ; Fax: 646-1710 Skype: boaz.shaanan
