Hello Amit,
Some great resources for general information are:
http://eagle.mmid.med.ualberta.ca/tutorials/HA/
Agniswamy et al, Acta Cryst D 64, 354
http://www.doe-mbi.ucla.edu/~sawaya/tutorials/Phasing/references.html
Recent threads also discussed halide soaks and Artem Evdokimov's
protocol for iodinating tyrosines.
We've also had success mutating residues to methionine followed by
SeMet phasing. I'd do this if the easy stuff doesn't work.
Ho
UC Berkeley
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Date: Mon, 6 Apr 2009 20:16:18 +0100
From: amit sharma <[email protected]>
Subject: soaking with Samarium chloride
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Dear All,
I have a crystal growing in the presence of 0.1M Sodium Acetate pH 5.0, 10%
PEG4000, 7.5% Dioxane and 10% Ethylene Glycol. I wanted to know if it would
be alright to use Samarium chloride for derivatization. I am worried about
the leaching of samarium by acetate. Also, what soak times can I use and
what conc. of Samarium chloride? My protein is 10 kDa , has no
cysteines/methionines and pI for the molecule is 4.6. Also, could somebody
suggest what other heavy atom soaks could be performed in this case?
Any suggestions would be appreciated.
Many thanks in advance
--
Amit Sharma
