Hi, The problem was the profile test failing as suggested by Wladek and Lothar (thanks to both, for continuous teaching since grad school). I was using a profile fitting radius of 25 (not the default 10), so that's why I assumed profile fitting was not the problem. But now, I have played with the profile fitting radius (PFR), and below are the numbers.
PFR Compl% Compl% (high res bin) 25 81 63 50 96 80 60 98 86 And with hindsight, it was very embarrassing to find in my log file "count of observations deleted due to zero sigma or profile test", which tells me the problem. And again, because mosflm was giving me 100% complete datasets, it had to be a scalepack-specific implementation. Interestingly, at a PFR of 50, a single profile covers almost a fourth of the image. Any input on that one? Engin ----- Original Message ----- From: "Wladek Minor" <[email protected]> To: [email protected] Sent: Saturday, April 11, 2009 5:11:40 PM GMT -08:00 US/Canada Pacific Subject: Re: [ccp4bb] HKL2000 and incompleteness Try to increase profile fitting radius. WM ------Original Message------ From: Ted Erickson Sender: CCP4 bulletin board To: [email protected] ReplyTo: Ted Erickson Subject: Re: [ccp4bb] HKL2000 and incompleteness Sent: Apr 11, 2009 7:56 PM >So before I lose it because of HKL2000, I have two questions, and I'd appreciate any answers to either: >1. Can there be another reason for incompleteness that I am missing (other than blind region at higher res., overlaps, overloads, and "not enough many frames")? Another explanation for incompleteness could be due to crystal packing that results in an asymmetric diffraction pattern. The discrepancy between HKL2000 and mosflm maybe a result of the resolution cutoff, however, this is only a speculation. Ted www.P212121.com Sent via BlackBerry by AT&T
