Hi,to help everyone, this observation (and similar ones) could (or maybe should) be added to others of its kind, in the CCP4 wiki at
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Conditions_prone_to_salt_crystallization HTH, Kay Jacob Keller schrieb:
Hi All,there is a condition in the JCSG+ screen (0.2M Li2SO4, 0.1M NaOAc pH 4.5, 50.0% v/v PEG-400) which forms heavy precipitate when made here in the lab. I assume that the order of addition of compounds might make a difference, but doesn't this suggest that there is a high probabilty for salt crystals under these conditions? How stable can this solution be? An anecdotes/advice appreciated.I assume that the PEG makes Li2SO4 fall out of solution, or perhaps it is the pH?Jacob Keller ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: [email protected] *******************************************
-- Kay Diederichs http://strucbio.biologie.uni-konstanz.de email: [email protected] Tel +49 7531 88 4049 Fax 3183 Fachbereich Biologie, Universität Konstanz, Box M647, D-78457 Konstanz This e-mail is digitally signed. If your e-mail client does not have the necessary capabilities, just ignore the attached signature "smime.p7s".
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