[ccp4bb] Lost my protein

[yanming Zhang]

Hi all,

during concentrating my protein, using Amicon Ultra centrifugal filter devices, 
5000g, 4C, I lost large amount of my protein (75%). I heard the same story from 
one of my colleagues too. It seems the membrane of the Amicon tube ate my 
protein. 
Why this happen and how to recover my protein? 

Thank you very much.

Yan