Re: [ccp4bb] Lost my protein

Tue, 05 May 2009 11:28:34 -0700

Hi Yanming,

I usually keep the follow through before I know nothing is in it. Also you may want to suspend your protein solution every 5-10 min to avoid precipitation because the concentration gradient forms rapidly during centrifugation. If all of these do not help, just try concentrators with different types of membrane.

Joe



Kornelius Zeth wrote: 
Hi Yanming,

you can try adding 1 M of urea, 1% detergent (OG,DM) that often helps to keep proteins in solution.

Best wishes

Kornelius

On Tue, 5 May 2009 09:06:09 -0700
 yanming Zhang <[email protected]> wrote:
  
Hi all,

during concentrating my protein, using Amicon Ultra centrifugal filter devices, 5000g, 4C, I lost large amount of my protein (75%). I heard the same story from one of my colleagues too. It seems the membrane of the Amicon tube ate my protein. 
Why this happen and how to recover my protein? 

Thank you very much.

Yan



      
    

 ----------------------------------------------
 Kornelius Zeth
 Max Planck Institute for Developmental Biology
 Dept. Protein Evolution
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 72076 Tuebingen, Germany
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 Fax -49 7071 601 349

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