>
> Dear all,
>
> One of my enzymes crystallized in 0.1 M citric acid pH 3.5 and 25% PEK
> 3350. The structure solution reveals, citrate molecule is occupying the
> active site.
> Moreover citrate mediates the dimer formation.
> I tried crystallization in the same condition with ligand using soaking
> with different concentrations of ligands and lower concentration of citric
> acid (below 0.5 M of citric acid, crystals are broken). Cocrystallization
> also tried.
> I collected several data sets with these combinations.
> The Structure solutions from all these data sets give only citrate complex.
>
> I found crystals only in citrate condition.
>
> Any suggestions !!
>
> Thanks.
> Skkannu
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