孙建 wrote:
Dear all:
Recently,I have a problem about molecular replacement with
identity 30% model. There are two moleculars in AU.Through
self-rotation analyze,there are a two-fold axis in AU along X axis or
Z axis.Then I rotate and translate the model with CNS MR module.
several models have been searched according self-rotation criterion.I
build the model using Phenix and Arp/wArp,but there are no good
results.The best result is R/Rfree 0.47/0.48.Also,I do MR with Phaser
and Molrep.The Phaser indicate Z-scores 6.5 and no good
results,neither do the Molrep.I am very confused about several
questions:
1.Just 30% identity model can be used for MR model?What at
least identity should be acquired for MR?
Hard to say. 30 or more than 20 should be on the edge. I think it should
depends on quality of your data and your model.
2.Are there good program editing the model except Chainsaw?
You can build your model manually by seaman or coot . Alternative
choice is mumbo.
3.How can I make sure my rotated and translated MR model is
right and can be used for next model building and refinement?
I don't know. I only test the solutions by refinement. The one ,which
can be refined , is the right one. Otherwise it is not.
Good luck,
leo
