Hey! I have found the FFAS server ( http://ffas.ljcrf.edu/ffas-cgi/cgi/ffas.pl ) to build the best models for low homology MR searches. A relevant paper: 2004 Acta Cryst D60 1229-1236.
Best of luck, Frank 2009/6/21 孙建 <sunjian00...@gmail.com> > Dear all: > Recently,I have a problem about molecular replacement with > identity 30% model. There are two moleculars in AU.Through > self-rotation analyze,there are a two-fold axis in AU along X axis or > Z axis.Then I rotate and translate the model with CNS MR module. > several models have been searched according self-rotation criterion.I > build the model using Phenix and Arp/wArp,but there are no good > results.The best result is R/Rfree 0.47/0.48.Also,I do MR with Phaser > and Molrep.The Phaser indicate Z-scores 6.5 and no good > results,neither do the Molrep.I am very confused about several > questions: > 1.Just 30% identity model can be used for MR model?What at > least identity should be acquired for MR? > 2.Are there good program editing the model except Chainsaw? > 3.How can I make sure my rotated and translated MR model is > right and can be used for next model building and refinement? > > > -- > Best > Wishes! > -- ============= Frank V. Murphy Work: 630-252-0680
