May be this could help,
*Protein Expression and Purification 45 (2006) 191–199*
* In vitro activation, purification, and characterization
of Escherichia coli expressed aryl-alcohol oxidase,
a unique H2O2-producing enzyme.
Francisco J. Ruiz-Dueñas, Patricia Ferreira, María J. Martínez, Angel T.
Martínez
*
an in-vitro refolding of a fungal 566 AA FAD-containing protein with FAD
added as refolding additive. We have managed to solve the structure.
Hope it helps.
--
PhD. Israel Sanchez Fernandez
EM-lab
Departamento de Ciencia de Proteinas
CIB-CSIC Madrid España
