Dear All, I am refining a structure of a complex between of 50kD protein and a 20kD glycosylated protien. The data is of 2.9 A resolution. The wilson B factor is as high as 86.3 A^2. The refinement seems well with R/Rf of 0.21/0.25. But the B-factor is extra high. For the 50kD part, the average B factor is 76.5 A^2. But the B factor of the 20 kD glycosylated protein is as high as 133.3 A^2. Although the electron density looks fine, even the sugar chain is seen clearly. My question is: 1. How to reduce the B factor to a reasonable level? 2. If it can not be redueced, when I published it, is this value acceptable? 3. In the same of similar resolutionIs, is there some other structures like this situation? A component or a subunit of the protein has a extra high B factor as high as 130.
Thanks and best wishes. -- Jiamu Du, Ph.D. State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue-Yang Road Shanghai 200031 P. R. China Tel: +86-21-5492-1117 E-mail: [email protected]
