Re: [ccp4bb] Weird expression behavior

[Ezra Peisach]

A little tangental....  You mentioned lowering the temperature - how 
low... Stratagene markets a call line - Arctic Express - that adds 
chaperones that are more active at lower temps.  I know someone who 
overcame inclusion body problems by expression at 16 using these cells. 
(I know someone else who regularly induces o/n at 16C w/ regular 
cells).  The only draw back is that these cells produce a protein that 
binds to NiNTA resin...

Good luck...