Hi Ezra and others,
Just thought I'd let you know that I have noticed that one or two of
those E. coli proteins that love to bind Ni-affinity resin do not
bind to Cobalt resin. Of course, there is always a price to pay (for
the Co resin, in this case) :)!
Cheers,
Raji
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Raji Edayathumangalam
Joint Research Fellow
Brigham and Women's Hospital/
Harvard Medical School
Brandeis University
On Sep 2, 2009, at 7:55 AM, Ezra Peisach wrote:
A little tangental.... You mentioned lowering the temperature -
how low... Stratagene markets a call line - Arctic Express - that
adds chaperones that are more active at lower temps. I know
someone who overcame inclusion body problems by expression at 16
using these cells. (I know someone else who regularly induces o/n
at 16C w/ regular cells). The only draw back is that these cells
produce a protein that binds to NiNTA resin...
Good luck...
