It might be risky (and silly), but I have done this successfully. I
just put a small dewar of liquid N2 under the scope, focussed
directly above the surface and looked to see if cryo solutions froze
clear or not. The ones that did worked out when we got to the
beamline (we'd verified that the crystals survived the treatment in
the solutions by eye as well). Only tried it that once, so I have no
idea how reliable it is.
Good luck,
Arthur Glasfeld
Department of Chemistry
Reed College
3203 SE Woodstock Blvd.
Portland, OR 97202
USA
On Sep 22, 2009, at 1:24 AM, Claudia Scotti wrote:
Dear List,
Sorry for the probably silly question.
Any suggestions to test cryoconditions without X-rays or cryostream?
I'd need to freeze crystals before going to ESRF and I'm a bit
anxious. Is it enough to try to freeze the cryoconditions in liquid
nitrogen checking under the microscope (or by eye) or is this still
risky?
Thanks,
Claudia
Claudia Scotti Dipartimento di Medicina Sperimentale Sezione di
Patologia Generale Universita' di Pavia Piazza Botta, 10 27100
Pavia Italia Tel. 0039 0382 986335/8/1 Facs 0039 0382 303673
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