or see concentrations given in:
McFerrin and Snell
J.Appl.Cryst (2002) 35, 538
and
Mitchell and Garman,
J.Appl.Cryst. (1996) 29, 584
Good luck
EFG
Paul Leonard wrote:
Hi Claudia
It really is best to test the cryo condition in an X-ray beam.
However, if you don't have access to X-rays in house you could look at the
hampton crystallization screen conditions (or another supplier). Find a
condition with a similar amount and type of precipitant as you have in
your crystallisation condition and look up how much glycerol they added as
cryoprotectant. Then try making up your own crystallisation condition
with the amount of glycerol they recommend and freeze your crystals in
that solution.
Also try crystal soaks of varying lengths of time (30 seconds - 5 min) in
the cryo condition as well as trying to soak some crystals where you
gradually increase the %glycerol in the crystal soak prior to freezing (in
2% glycerol steps for example).
see
http://hamptonresearch.com/product_detail.aspx?cid=1&sid=20&pid=3
Good luck.
Paul
Dear List,
Sorry for the probably silly question.
Any suggestions to test cryoconditions without X-rays or cryostream?
I'd need to freeze crystals before going to ESRF and I'm a bit anxious. Is
it enough to try to freeze the cryoconditions in liquid nitrogen checking
under the microscope (or by eye) or is this still risky?
Thanks,
Claudia
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