Hello everyone,
I am no structural biologist but a microbiologist. I have a cooperation with a
crystallographic group to solve the structure of a bacterial enzyme. Now we
have recorded a dataset and are solving the structure. My problem is that the
whole Structural Biologist thing is so complex (Biology, chemistry physics...).
Nevertheless its very fascinating. But what I want to know is, if there is a
good textbook for it. I got "crytallography made crystal clear" but this book
isn't enough at all. Especially for data processing. I want to know things like
which values are good for Rfactor observerd/expected or for R-meas Rmrgd-F
after the correctstep with xds. Is 80% completness still ok? And after scaling
we had a Rmerge of 0.156 (overall; at 2.2 A).
My pHD-cooperation partner isn't that experienced as well, so its difficult to
understand everything and do everything correct.
I hope these questions are ok and somebody can help with a textbook or
publication.
Thanks in advance,
Katja