Hi Maria,
maybe SeMet incorporation is not something they do in
Grenoble per se, but they do similar experiments on their MALDI-TOF
very competently (I guess you contacted Eric Forest's group?
http://www.ibs.fr/Plates-formes/Caracterisation-de-proteines/Spectrometrie-de-Masse/
.They have an Applied Biosystems Voyager if I recall correctly.)
In Paris (Orsay, IBBMC the institute where I am) there is also
Christophe Marchand, who has an almost identical instrument and does
SeMet incorporation experiments regularly.
http://www.ibbmc.u-psud.fr/spip.php?rubrique41
Try 2 samples- one of your protein alone, and one of a mix of native
protein mixed with SeMet labelled protein- you should have a credible
mass difference between the 2 peaks, and that gives you a nice
internal standard.
Good luck.
Mark
P.S. Contact me off-list if you need more info.
2009/9/28 Marija Backovic <[email protected]>:
> Dear all -
>
> Does anyone know of a facility that provides amino acid analysis for
> determination of SeMet incorporation in a recombinant protein?
>
> I contacted centers at Grenoble and Uppsala, but SeMet determination is not
> something they routinely do.
>
> Any suggestions would be highly appreciated!
>
> Best,
>
> Marija
>
>
> Marija Backovic, Ph.D.
>
> Pasteur Institute
> Department of Virology
> 25 rue du Dr Roux
> 75015 Paris
> France
>
> +33 (0)1 45 68 82 87
>
--
Mark Brooks, IBBMC, UMR8619 - Bâtiment 430,
Université de Paris-Sud, 91405 Orsay, France.
Tel: (33) 169157968
Fax: (33) 169853715
Skype: markabrooks
