Dear Marija
This can be done by Proton Induced X-ray Emission on a tiny sample
(0.2microl) of liquid protein to get the sulphur to selenium ratio and
thus work out the substitution of Selenium.
See:
http://www.ionbeamcentre.co.uk/index.php?target=3:68
and contact
Dr. Chris Jeynes for this service:
[email protected]
Best wishes
Elspeth
Marija Backovic wrote:
Dear all -
Does anyone know of a facility that provides amino acid analysis for
determination of SeMet incorporation in a recombinant protein?
I contacted centers at Grenoble and Uppsala, but SeMet determination
is not something they routinely do.
Any suggestions would be highly appreciated!
Best,
Marija
Marija Backovic, Ph.D.
Pasteur Institute
Department of Virology
25 rue du Dr Roux
75015 Paris
France
+33 (0)1 45 68 82 87
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******NOTE NEW E-MAIL ADDRESS AND PHONE NUMBER************
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Elspeth F. Garman,
President, British Crystallographic Association
Professor of Molecular Biophysics, University of Oxford
Visiting Professor in Chemistry, University of Durham
Postal address:
Laboratory of Molecular Biophysics,
Department of Biochemistry,
University of Oxford, Tel: (44)-1865-613297
South Parks Road, FAX: (44)-1865-613201
OXFORD, OX1 3QU, U.K. E-mail: [email protected]
http://www.biop.ox.ac.uk/www/garman/gindex.html
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