Re: [ccp4bb] B values bottomed out

[Eleanor Dodson]

Are you using TLS?
If so I usually start with all Bs reset to the Wilson B ( see GUI) It 
can still happen but not so often..
Eleanor#

Gloria Borgstahl wrote:
Hello ccp4-ers,

We are refining a 1.55 angstrom cryocooled structure with REFMAC.
In analyzing the occupancy of ligands in the active site we noticed
that all the residues there have bottomed out with an isotropic B value of 
2.0.
There is residual Fo-Fc density as the B values are not refined well.
How can one fix this?

Thanks, Gloria