I'll second this. We've done this as an exercise in NSLS Membrane
Protein Crystallization workshop for a few years, and it works like a
charm. You can stain in a warm iodine chamber and visualize by
scanning the TLC plate on a garden variety scanner (we use an
inexpensive Canon LIDE that probably cost less than USD 60 five years
ago). We quantify the spot intensity with NIH Image or equivalent, and
get lovely linearity down to the CMC, spotting only 1 uL of sample--so
we haven't seen any need to concentrate.
On 23 Oct 2009, at 3:41 PM, Edward A. Berry wrote:
Only easy if you happen to have silica gel TLC plates and
a chromatography jar lying around, perhaps from some
phospholipid analysis:
A strategy for identification and quantification of
detergents frequently used in the purification of membrane proteins
Laura R. Eriks, June A. Mayor, and Ronald S. Kaplan
Analytical Biochemistry 323 (2003) 234–241
This paper recommends spotting on a TLC plate and running
beside standard amounts of the same detergent. From intensity/size
of the detergent spot after developing you can bracket the detergent
concentration. (And by the way they found that detergents are
concentrated by ultrafiltration). To increase sensitivity, speedvac
a volume too large to
spot on the plate, dissolve the residue in Me0H.
Ed
[email protected] wrote:
Hi Folks:
After concentrating a membrane protein, is there a (easy) way of
measuring
the detergent concentration in the sample?
Regards,
Weikai
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Director, Biochemistry Graduate Program
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